Description
B. Paul Morgan and a team of expert laboratorians present a comprehensive set of readily reproducible methods to study this critical system. These cutting-edge techniques are suitable both for the basic scientist interested in understanding complement’s mechanisms of activation and for the clinical scientist wishing to quantify its activation, and range from the purification of its components to generating complement-deficient mice by gene deletion. Additional techniques presented include procedures for the analysis of its function, for the study of its regulators, for detection of its activation in vivo, and for the identification of its autoantibodies. Comprehensive and cutting-edge, Complement Methods and Protocols offers today’s basic and clinical investigators powerful tools for the analysis of the role of complement in human pathophysiology and disease, as well as its therapeutic regulation. The Complement System: An Overview, B. Paul Morgan. Purification of Complement Components, Regulators, and Receptors by Classical Methods, Carmen W. van den Berg. Immunoaffinity Methods for Purification of Complement Components and Regulators, B. Paul Morgan. Measurement of Complement Hemolytic Activity, Generation of Complement-Depleted Sera, and Production of Hemolytic Intermediates, B. Paul Morgan. Measurement of Complement Lysis of Nucleated Cells, O. Brad Spiller. Functional Assays for Complement Regulators, Claire L. Harris. Immunochemical Measurement of Complement Components and Activation Products, Reinhard Wrzner. Complement Deposition in Tissues, Antti Vkev and Seppo Meri. Complement Regulators and Receptors in Tissues, Juha Hakulinen and Seppo Meri. Measurement of C3 Fragment Deposition on Cells, O. Brad Spiller. Screening for Complement Deficiency, Ann Orren. C1-Inhibitor: Antigenic and Functional Analysis, C. Erik Hack. Autoantibodies to Complement Components, Kevin A. Davies and Peter Norsworthy. Allotyping of Complement Components, Reinhard Wrzner. Complement and Immune Complexes, Julian T. Nash and Kevin A. Davies. Knocking Out Complement Genes, Anne E. Bygrave and Marina Botto. Inherited Complement Deficiencies in Animals, Stuart Linton. Appendices. Suppliers. Sources of C Components and Anti-C Antibodies. cDNA Accession Numbers for C Components. Regulators, and Receptors. Index.
